Recent Covers

Efficacy of an organophosphorus hydrolase enzyme (OpdA) in human serum and minipig models of organophosphorus insecticide poisoning

Eddleston M, Clutton ERTaylor MThompson AWorek FJohn HThiermann H & Scott C

Clinical Toxicology

One-pot multi-enzymatic transformation of NH3, CO2 and ornithine into the organic plant fertilizer citrulline using a single recombinant lysate of E. coli

Alissandratos A, Hartley CJ, French NG, Kim HK, Allen S, Estavillo GM, Scott C & Easton CJ.

Sus Chem Eng.

A revised biosynthetic pathway for the cofactor F420 in prokaryotes

Bashiri G, Antoney JP, Jirgis ENM, Shah MV, Ney B, Copp J, Stutely SM, Sreebhavan S, Palmer B, Middleditch M, Tokuriki N, Greening C, Scott C, Baker EN, & Jackson CJ

Nat. Comms. 10:  1558

PREPRINT: Engineered Enzymes that Retain and Regenerate their Cofactors Enable Continuous-Flow Biocatalysis

Hartley CJ, Williams CC, Scoble JA, Churches QI, North A, French NG, Nebl T, Coia G, Warden AC, SImpson G, Frazen A, Nixon Jensen C, Turner NJ & Scott C



A novel decarboxylating amidohydrolase involved in avoiding metabolic dead ends during cyanuric acid catabolism in Pseudomonas sp. strain ADP.

Esquirol L, Peat TS, Wilding M, Hartley CJ, Newman J & Scott C.

PLoS One 13: e0206949

Isolation of the (+)-pinoresinol-mineralizing Pseudomonas sp. strain SG-MS2 and elucidation of its catabolic pathway

Shettigar M, Balotra S, Cahill D, Warden AC, Lacey ML, Kohler HPE, Rentsch D, Oakeshott JG & Pandey G

Appl. Environ. Microbiol. 84: e02531

Computer-guided surface engineering for enzyme improvement.

Wilding M, Scott C & Warden AC

Sci. Report 8: 11998

An unexpected vestigial protein complex reveals the evolutionary origins of an s-triazine catabolic enzyme.

Esquirol L, Peat TS, Wilding M, Liu JW, French NG, Hartley CJ, Onagi H, Nebl T, Easton CJ, Newman J & Scott C

J. Biol. Chem - Editor's Pick (Old enzymes versus new herbicides)

Structural and biochemical characterization of the biuret hydrolase (BiuH) from the cyanuric acid catabolism pathway of Rhizobium leguminosarum bv. viciae 3841.

Esquirol L, Peat TS, Wilding M, Lucent D, French NG, Hartley CJ, Newman & Scott C.

PLoS One 13: e0192736.

Hyperthermophilic carbamate kinase stability and anabolic in vitro activity at alkaline pH.

Hennessy JE, Latter MJ, Philbrook A, Bartkus D, Kim HK, Oakeshott JG, Scott C, Alissandratos A & Easton CJ. 

Appl. Environ. Microbiol. 84: e02250-17


Reverse engineering: transaminase biocatalyst development using ancestral sequence reconstruction.

Wilding M, Peat TS, Newman J, Scott C & Jermiin LS

Green Chem. 19: 5375-5380


Sugar analog synthesis by in vitro biocatalytic cascade: a comparison of alternative enzyme complements for dihydroxyacetone phosphate production as a precursor to rare chiral sugar synthesis.

Hartley CJ, Scoble JM, Williams CC, Quentin QI, Frazer A, French NG, Taylor MC, Coia G, Simpson G, Turner NJ & Scott C

PLoS One 12: e0184183.


An improved and general streamlined phylogenetic protocol applied to the fatty acid desaturase family.

Wilding M, Nachtschatt M, Speight R & Scott C

Mol. Phylogenet. Evol. 115: 50-57


F420H2-dependent reductases promiscuously reduce diverse compounds through a common hydrogenation mechanism.

Greening C, Jirapanjawat T, Afroze S, Ney B, Scott C, Pandey G, Lee BM, Russell RJ, Jackson CJ, Oakeshott JG, Taylor MC & Warden AC

Frontiers in Microbiol. 8: 1000 

Cofactor tail length modulates catalysis of bacterial F420-dependent oxidoreductases

Ney B, Carere CR, Sparling R, Jirapanjawat T, Stott MB, Jackson CJ, Oakeshott JG, Warden AC & Greening C

Frontiers in Microbiol 8: 1902

Design of silk proteins with increased heme binding capacity and fabrication of silk-heme materials

Rapson TD, Liu JW, Sriskantha A, Musameh M, Dunn CJ, Church JS, Woodhead A, Warden AC, Riley MJ, Harmer JR, Noble CJ & Sutherland TD

J. Inorgan. Biochem. 177: 219-227


High resolution X-ray structures of two functionally distinct members of the cyclic amide hydrolase (CyAH) family of Toblerone fold enzymes

Peat TS, Balotra S, Wilding M, Hartley CJ, Newman J & Scott C

Appl. Environ. Microbiol. e03365-16


Hacking nature: genetic tools for reprogramming enzymes.

Hartley CJ, Wilding M & Scott C

Microbiol. Austral. 38: 73-53

A Motif in the F Homomorph of Rabbit Haemorrhagic Disease Virus Polymerase Is Important for the Subcellular Localisation of the Protein and Its Ability to Induce Redistribution of Golgi Membranes

Urakova N, Warden AC, White P, Strive T, Frese M

Viruses 9: 202

Comparative lipidomics and proteomics of lipid droplets in the mesocarp and seed tissues of Chinese tallow (Triadica sebifera)

Zhi Y, Taylor MC, Campbell PM, Warden AC, Shrestha P, Tahchy AE, Rolland V,  Vanhercke T, Petrie JR, White RG, Chen W, Singh SP & Liu Q

Frontiers in Plant Science 8: 1339


X-ray crystal structure of a malonate semialdehyde dehydrogenase from Pseudomonas sp. strain AAC.

Wilding M, Scott C, Newman J & Peat TS

Acta Cryst F 73: 24-28.


Crystal structure of a putrescine aminotransferase from Pseudomonas sp. strain AAC.

Acta Cryst F 73: 29-35


Epistasis tightly constrains the addition of a catalytic ionizable residue and the divergence of enzymatic function in triazine hydrolases.

Sugrue E, Scott C & Jackson CJ

Organic Biomol. Chem. 15: 937



F420 and F0 dependent redox reactions: physiology, biochemistry, and applications.

Greening C, Ahmed FH, Mohamed AE, Lee BM, Pandey G, Warden AC, Scott C, Oakeshott JG, Taylor MC & Jackson CJ

Microbiol. Mol. Biol. Review. 80: 451-493


Molecular mechanisms of evolution towards xenobiotic hydrolysis in bacterial metalloenzymes.

Sugrue E, Hartley CJ, Scott C & Jackson CJ

Aust. J. Chem. 69: 1383-1389

Bacterial biodegradation of neonicotinoid pesticides in soil and water systems
Hussain S, Hartley CJ, Shettigar M, and Pandey G
FEMS Microbiol. Lett. 363: fnw252


A β-alanine catabolic pathway containing a highly promiscuous ω-transaminase from the 12-aminododecanate-degrading Pseudomonas sp. Strain AAC.

Wilding M, Peat TS, Newman J & Scott C

Appl. Environ. Microbiol.82: 3846-3856.


Active site desolvation and thermostability trade-offs in the evolution of catalytically diverse triazine hydrolases.

Sugrue E, Carr PD, Scott C & Jackson CJ.

Biochemistry. 55: 6304–6313


X-Ray structure and mutagenesis studies of the N-isopropylammelide isopropylaminohydrolase, AtzC.

Balotra S, Warden AC, Newman J, Briggs LJ, Scott C & Peat TS

PLoS One 10: e0137700.


Evolutionary expansion of the amidohydrolase superfamily in response to synthetic compounds: molinate and diuron hydrolases.

Sugrue E, Fraser NJ, Hopkins DH, Carr PD, Khurana JL, Oakeshott JG, Scott C & Jackson CJ

Appl. Environ. Microbiol. 81: 2612-2625

The structure of the hexameric atrazine chlorohydrolase, AtzA.

Peat TS, Newman J, Balotra S, Lucent D, Warden AC & Scott C

Acta Cryst D. 71:710-720


Isomer-specific comparisons of the hydrolysis of synthetic pyrethroids and their fluorogenic analogues by esterases from the cotton bollworm Helicoverpa armigera.

Yuan G, Li YQ, Farnsworth CA, Coppin CW, Devonshire AL, Scott C, Russell RJ, Wu YD & Oakeshott JG

Pesticide Biochem. Physiol. 121: 102-106.


Identification of novel transaminases from Pseudomonas sp. strain ACC.

Wilding M, Walsh E, Dorrian S & Scott C

Microbial Biotechnol 8: 665-672.


X-ray structure of the amidase domain of AtzF, the allophanate hydrolase from the cyanuric acid-mineralizing multienzyme complex.

Balotra S, Newman J, Cowieson N, French NG, Campbell PM, Briggs LJ, Warden AC, Easton CJ, Peat TS & Scott C

Appl. Environ. Microbiol. 81: 470-480



300-Fold increase in production of the Zn2+-dependent dechlorinase TrzN in soluble form via apoenzyme stabilization.

Jackson CJ, Coppin CW, Carr PD, Aleksandrov A, Wilding M, Sugrue E, Ubels J, Paks M, Newman J, Peat TS, Russell RJ, Field M, Weik M, Oakeshott JG & Scott C

Appl. Environ. Microbiol. 80: 4003-4011


A 5000-fold increase in the specificity of a bacterial phosphotriesterase for malathion through combinatorial active site mutagenesis.

Naqvi T, Warden AC, French NG, Sugrue E, Carr PD, Jackson CJ & Scott C

PLoS One 9: e94177


Crystallization and preliminary X-ray diffraction analysis of the amidase domain of allophanate hydrolase from Pseudomonas sp. strain ADP.

Balotra S, Newman J, French NG, Briggs LJ, Peat TS & Scott C

Acta Cryst. F. F70:1-6.


Ongoing functional evolution of the bacterial atrazine chlorohydrolase AtzA.

Noor S, Changey F, Oakeshott JG, Scott C & Martin-Laurent F

Biodeg. 25: 21-30



Cyanuric acid hydrolase: evolutionary innovation by structural concatenation.

Peat TS, Balotra S, Wilding M, French NG, Briggs LJ, Panjikar S, Cowieson N, Newman J & Scott C

Mol. Microbiol. 88: 1149-1163


Heterologous expression and biochemical characterisation of fourteen esterases from Helicoverpa armigera.

Teese MG, Farnsworth CA, Li YQ,.Coppin CW, Devonshire AL, Scott C, East P, Russell RJ & Oakeshott JG

PLoS One 8: e65951


How many genetic options for evolving insecticide resistance in heliothine and spodopteran pests?

Oakeshott JG, Farnsworth CA, East PD, Scott C, Han Y, Wu Y & Russell RJ

Pest. Manag. Sci. 69: 889-896


Organophosphate and pyrethroid hydrolase activities of mutant esterases from the cotton bollworm Helicoverpa armigera.

Li Y, Farnsworth CA, Coppin CW, Teese MG, Liu JW, Scott C, Zang X, Russell RJ & Oakeshott JG

PLoS One 8: e77685


Determination of the structure of the catabolic N-succinylornithine transaminase (AstC) from Escherichia coli.

Newman J, Seabrook S, Surjadi R, Williams CC, Lucent D, Wilding M, Scott C & Peat TS

PLoS One 8: e58298


F420-Dependent Enzymes – Potential for Applications in Biotechnology.

Taylor MC, Scott C & Grogan G

Trends Biotechnol. 31: 63-64​

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